Duval County, FL Businesses for Sale | Buy or Invest Today

Explore Duval County businesses for sale on DealStream and discover prime business opportunities in Jacksonville, FL. Our curated listings feature everything from thriving retail shops and restaurants to service-based ventures and profitable franchises, complete with detailed financials and location insights. Use powerful search filters to compare listings and connect directly with sellers to secure your ideal business investment. Start browsing today and turn your dream of business ownership in Duval County, Florida into reality.

All Matching Deals

PCR Lab, High-Complex CLIA with New PTAN
Jacksonville, FL

Fully CLIA Licensed / Credentialed and molecular diagnostics laboratory in the Non-MolDX mac, North Florida area. This turnkey facility is built for high-throughput PCR testing with a complete workflow setup, ready for immediate operation. Owners are seeking $210k for an outright acquisition of all...

$210,000
Details
Jacksonville, FL

This portable restroom trailer provider delivers premium restroom units for outdoor weddings, festivals, private celebrations, corporate gatherings and construction projects where clients want a cleaner, more comfortable experience than standard porta potties. The business focuses on upscale trailers...

$259,000
Cash Flow: $237,374
Details
Jacksonville, FL

Discover a rare opportunity to acquire a fully fenced, ±0.8-acre property featuring a 2,700 sq. ft. stand-alone building in a sought-after IL-zoned corridor. This versatile site works perfectly for an owner-operator, investor, or expansion-minded buyer seeking a property with some cash flow already...

$750,000
Cash Flow: $43,923
Details
Jacksonville, FL

Here is the chance to take over these TWO sports bar locations for far less than the asset value! Both locations have indoor and outdoor seating, long-term leases, and full bars (4 COP SFS licenses) in place. Current owners are not in the business day-to-day; this is an ideal opportunity for an active...

$500,000
Details
Jacksonville, FL

This established and locally-operated waste management company offers junk removal, recycling, demolition, and dumpster rental services tailored for both residential and commercial clients—including offices, retail spaces, and construction sites. Built on a recession-resistant model, this business...

$138,500
Cash Flow: $186,550
Details
Great Pool Service Route
Jacksonville, FL

Nice pool route with 60 customers, of which two are commercial accounts. Business also performs equipment repair for additional revenue. The business includes a database of homeowners who have pools for business development. Business has one full-time 1099 employee, but can be run by the owner if they...

$75,000
Cash Flow: $40,000
Details
278-Door Property Management Franchise
Jacksonville, FL

Thriving franchise property management company operating in South Florida. Established 7 years, the current owner has owned it for 3 years. They grew the business from 40 doors to 400 with 278 (and counting) currently active. Business assists homeowners in updating properties, leasing, legal/evictions,...

$1,200,000
Cash Flow: $325,474
Details
Jacksonville, FL

Practice Fact Sheet: Jacksonville, Florida Total Gross Income: $953,735 Net Profit: $618,290 includes Owner add backs Purchase Price: $1,239,000– Financing available to qualified applicants. Various programs available. Call for details. Year Established: 1998 Accounting, bookkeeping, Compilations...

$1,239,000
Cash Flow: $618,290
Details
Over 50 Year-Old Millwork Business w/ Real Estate
Duval County, FL

If you are looking for a long-standing business with growth opportunities, this manufacturing-adjacent business may be the opportunity for you. There are currently 5 employees, but Sellers feel strongly that doubling the employee count could nearly triple the current business. There is no advertising...

$1,512,000
Cash Flow: $301,972
Details
2 Established Jacksonville Drop Stores
Duval County, FL

This sale includes 2, well-established drop stores. Seller estimates drop store 1 grosses ~$105k/year and drop store 2 grosses ~$85k/year currently. Liberty POS used in both locations. Drop Store 1: 1,000 square feet / $1,802.08 all-in/month Drop Store 2: 800 square feet / $1,365.33 all-in/month Locations...

$155,000
Details
Molecular Laboratory For sale (Non-MolDx)
Jacksonville, FL

Fully CLIA Licensed / Credentialed and molecular diagnostics laboratory in the Non-MolDX mac, North Florida area. This turnkey facility is built for high-throughput PCR testing with a complete workflow setup, ready for immediate operation. Owners are seeking $190k for an outright acquisition of all...

$190,000
Details
Non-MolDX Molecular Laboratory for sale
Jacksonville, FL

Fully CLIA Licensed / Credentialed and molecular diagnostics laboratory in the Non-MolDX mac, North Florida area. This turnkey facility is built for high-throughput PCR testing with a complete workflow setup, ready for immediate operation. No testing or billing has occurred. Owners are seeking $195k...

$195,000
Details
High-Complex CLIA PCR Lab in FL
Jacksonville, FL

High-Complex CLIA Certified, Fully Credentialed Molecular laboratory for sale in FCSO mac. It has the following set up and is turn key for testing and billing: SPOT Billing portal set up with FCSO, Medical Director, Technical Supervisor, PCR Instrument, -20C Freezer for PCR reagent, Fridges, Tables,...

$230,000
Details
North Florida Molecular laboratory
Jacksonville, FL

High-Complex CLIA Certified, Fully Credentialed Molecular laboratory for sale in FCSO mac. It has the following set up and is turn key for testing and billing: SPOT Billing portal set up with FCSO, Medical Director, Technical Supervisor, PCR Instrument, -20C Freezer for PCR reagent, Fridges, Tables,...

$220,000
Details
Jacksonville, FL

This device has been cleared by FDA and ready to market Everything is in order in terms of manufacturing and commercialization of this device Similar device has produced millions in sales Used for Psoriasis with Hugh target market

On Request
Details
Duval County, FL

Opportunity to acquire an established franchise-based outdoor services business operating in a highly attractive and protected territory in Northeast Florida. The company has been in operation for three years and is demonstrating continued growth, supported by strong demographics, repeat customers,...

$449,000
Cash Flow: $170,069
Details
North Florida Roofing and Remodeling Contractor
Duval County, FL

This is a rare opportunity to acquire a highly regarded roofing and home remodeling contractor located in North Florida. The company specializes in residential remodeling and roofing services, providing quality workmanship and reliable service to its clients. The current owner is committed to ensuring...

$1,100,000
Cash Flow: $243,482
Details
Jacksonville, FL

Great opportunity to acquire an owner-operated shipping and packaging business located in Jacksonville, Florida. The store was opened approximately one year ago and has already established a solid operational framework. While still in the early stages of development, the business model is streamlined,...

$125,000
Cash Flow: $104,000
Details
Duval County, FL

This is a uniques opportunity to acquire a reliable and convenient drug testing franchise with two established locations. The business provides a wide range of lab testing services. Both labs are strategically located in high-demand areas, offering customers easy access and consistent, professional...

$100,000
Details
Duval County, FL

This popular neighborhood ice cream and dessert shop has been delighting customers for over 15 years with a variety of frozen treats, beverages, and snack offerings. Located in a high-traffic retail corridor in Jacksonville, FL, the shop benefits from strong visibility, convenient access, and a loyal...

$150,000
Cash Flow: $28,666
Details
Duval County, FL

This established Jacksonville business specializes in the high-demand niche of engine block and cylinder head welding and repair. It is an asset-rich opportunity, featuring approximately $210,000 in specialized machining equipment and Commercial Real Estate. By focusing on precision repairs that most...

$670,000
Cash Flow: $105,979
Details
Duval County, FL

Imagine walking into your own thriving auto repair business where everything is already running smoothly. This well-established, big brand auto repair and tire service center in Jacksonville's BOOMING DUVAL County offers exactly that - a turnkey opportunity you'll love. You'll appreciate the prime...

$489,000
Cash Flow: $105,712
Details
Jacksonville FL Chiropractic Patient File Sale
Jacksonville, FL

Excellent opportunity to continue care for an established patient base that already values chiropractic. Clinics that acquire existing patient files often see an immediate lift in visits and revenue, as patients prefer to stay with providers familiar with their case history. This transfer offers a streamlined...

On Request
Details
Jacksonville, FL

NEW Turnkey Shopify Startup Website Business. The website comes programmed with products and suppliers to drop ship the products for you. No programming skills or experience is required to profit from this highly lucrative industry.There is no inventory to carry and nothing to ship. You can own and...

$799
Details
Duval County, FL

Where Beauty Meets Excellence!Step into a sanctuary of self-care, where every detail is designed with you in mind. Whether you're looking to revamp your hairstyle, treat your nails to a little luxury, or indulge in a rejuvenating facial, we've got every service you need to feel flawless from head to...

$99,000
Cash Flow: $75,258
Details

Tips for Buying A Business In Duval County, FL

Understand the Local Market Dynamics

Before purchasing a business in Duval County, FL, it’s crucial to thoroughly research and understand the local market dynamics. Jacksonville, being both the urban and commercial hub of Duval County, has unique economic drivers such as logistics, healthcare, tourism, and technology. Analyze local demographics, consumer trends, and the competitive landscape to ensure the business you are considering aligns with current and future market demands. Connect with local chambers of commerce, attend industry events, and consult market reports to gather comprehensive insights.

Conduct Comprehensive Due Diligence

One of the most important steps when buying a business is conducting exhaustive due diligence. In Duval County, pay attention to local licensing, zoning regulations, and environmental requirements that may impact operations. Carefully review the financial statements, contracts, leases, and legal history of the business. It’s also wise to meet with key employees and customers to gauge the business’s reputation and operational health. Engage local legal and financial professionals who are familiar with regional business laws to help you avoid unexpected liabilities.

Leverage Local Relationships and Resources

Success in buying and running a business in Duval County often hinges on building strong local relationships. Establish connections with local bankers, suppliers, and advisors who understand the area’s business climate. Take advantage of support from regional small business development centers, networking groups, and business incubators. These local resources can provide valuable mentorship, financing options, and operational support as you transition into ownership and work to grow your new enterprise.